Part:BBa_K112801:Design

{mRFP2}{mRFP2} Red Fluorescent Protein with start and Stop

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 100
  

Design Notes

atgatatttgatgctagtttaaagAAGTTGAGGAAATTATTTGTAAATCCAATTGGGTTTTTCCGTGACTCATGGTTTTTTAATTCTAAAAACAAGGCTGAAGAGCTACTATCACCGTTAAAAATAAAAAGTAAAAATATTTTTATAATTAGTAACCTGGGGCAATTAAAAAAAGCTGAGTCATTTGTACAAAAATTTAGCAAGAGAAGTAACTATCTTATTGTTTTGGCAACTGAAAAAAATACTGAGATGCCAAAAATTATTGTTGAACAAATAAATAATAAATTATTTTCTTCATACAAGGTACTATTCATTCCAACTTTCCCAAATGTTTTTTCACTTAAAAAGGTTATATGGTTTTATAACGTATATAATTATTTAGTTTTAAATTCAAAAGCTAAAGATGCTTATTTTATGAGCTATGCGCAACATTATGCAATCTTCGTATATTTGTTCAAAAAAAATAATATAAGATGTTCATTAATTGAAGAGGGGACAGGGACTTATAAAACCGAAAAAGAAAACCCAGTAGTAAATATTAATTTTTATTCAGAGATTATTAATTCAATTATCTTGTTCCATTATCCAGATTTGaaatttgaaaatgtatacggtacatatccaattttgcttaagaaaaaatttaatgcgcaaaaatttgttgagtttaaaggtgctccatcagttaaatcatcaaccagaatagataatgttatccataaatattctataactagagatgatataatatatgcaaatcaaaagtatttgattgaacatacattatttgcggATTCGTTAATTTCTATCTTACTTAGAATAGATAAGCCTGATAATGCAAGAATATTTATAAAACCTCACCCTAAAGAGCCTAAAAAAAATATTAATGCAATTCAAAAGGCAATAAAAAAGGCAAAATGTCGTGACATAATTCTTATAACAGAGCCAGACTTTTTAATAGAGCCGGTAATAAAAAAAGCAAAAATAAAACACTTAATTGGATTAACATCATCTTCTTTGGTATATGCACCTTTAGTTTCTAAAAGATGTCAGTCTTATTCAATAGCGCCTCTTATGATAAAGTTGTGTGATAATGATAAATCCCAAAAAGGGATTAATACGCTGCGTCTCCATTTCGATATTTTAAAGAATTTTGATAATGTTAAAATATTATCGGATGATATAACATCTCCCTCTTTGCACGATAAAAggattttcttgggggagtaa

Source

Constructing the pSB1A2 variant (pBca9145) and mRFP basic part (Bca1089)

PCR ca1089F/R on I13521         (~780 bp, EcoRI/PstI)

Sub into pSB1A2-I13521          (EcoRI/PstI)

Product is pBca9145-Bca1089